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Subject: Fluorescence measurement system

Date: 04/18/02 at 9:07 PM
Posted by: Ryan O'Neill
E-mail: zombiebeat@go.com
Message Posted:

Hi ,
I'm designing a floursecence measurement system for detection of oxygen in seawater.

So far I've decided to go with a Blue LED(this excites the Ruth(II) complex im using at 470nm] a bifurcate fibre bundle connected to my chemical sensor(the Ruth(II)complex) it also houses a RTD temperature sensor.

The fibre bundle leads to a Photomultiplier tube (voltage regulated) and then a lock in amp and finally my data display (laptop)

I know I need to add on lenses and filters.

This is where I'm a little stuck 2 bandpass filters? One at the LED one at the PMT?

I'm really confused on lenses I think one lens at the PMT to focus the incoming protons?

I've searched textbooks and the web and I can't find information which will help me decide on the correct filters and lenses.

Thankyou for your time, any help appreciated.

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