In Reply to: Re: Fluorescence measurement system posted by Mike Pagano on 04/19/02 at 12:11 PM:
Thanks for the reply Mike,
I ve been working on the filter problem. I see you recommended a notch or a narrow band filter, I explored this option;
The normal Ruth(II) complex lamda is from 350 nm to 600 nm
The "excited" state 500 -700+nm.
So a good peak for the normal is 470nm which my LED excites at.
The detection of the excited state is a sharp peak at 600nm so I am looking for a filter that cuts off all below this (as this will negate any interference of the normal Ruth(II)
I have been looking at a visible long pass filter to do this(on the PMT), it has a cut-on of 600nm, a blocking range of low UV 560 and a transmission range of 605-2000. These figures are from my Ealing catalog
I think a band-pass filter would be ideal for my LED->Optical cable to ensure only the required lamda is entering the cable.
Again thanks for any advice you can offer, will I have to employ lenses?