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Subject: Re: Fluorescence measurement system

Date: 04/25/02 at 7:39 PM
Posted by: Ryan O'Neill
E-mail: zombiebeat@go.com
Message Posted:

In Reply to: Re: Fluorescence measurement system posted by Mike Pagano on 04/19/02 at 12:11 PM:

Thanks for the reply Mike,

I ve been working on the filter problem. I see you recommended a notch or a narrow band filter, I explored this option;

The normal Ruth(II) complex lamda is from 350 nm to 600 nm

The "excited" state 500 -700+nm.

So a good peak for the normal is 470nm which my LED excites at.

The detection of the excited state is a sharp peak at 600nm so I am looking for a filter that cuts off all below this (as this will negate any interference of the normal Ruth(II)

I have been looking at a visible long pass filter to do this(on the PMT), it has a cut-on of 600nm, a blocking range of low UV 560 and a transmission range of 605-2000. These figures are from my Ealing catalog

I think a band-pass filter would be ideal for my LED->Optical cable to ensure only the required lamda is entering the cable.

Again thanks for any advice you can offer, will I have to employ lenses?


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